Journal: PLoS ONE

Article Title: Construction of the experimental rat model of gestational diabetes

doi: 10.1371/journal.pone.0273703

Figure Lengend Snippet: (A) Effects on the protein expression levels of GLUT1 and GLUT3 and the internal reference β-actin as determined by Western blot analysis, (B) Comparison of the protein expression levels of GLUT1 and GLUT3. * P < 0.05 vs. the NC group; # P < 0.05 vs. the HFHS group.

Article Snippet: After blocking with 5% skimmed milk in PBS-Tween, the membranes were probed with anti-glucose transporter 1 (GLUT1) or anti-glucose transporter 1 (GLUT3) primary antibody (1:500, Boeter) at 4°C overnight and conjugated with secondary antibodies at room temperature for 45 min. Antibodies against GLUT1 and GLUT3 were obtained from BOSTER Biological Technology Co., Ltd.

Techniques: Expressing, Western Blot, Comparison

Journal: PLoS ONE

Article Title: Acquired Alterations of Hypothalamic Gene Expression of Insulin and Leptin Receptors and Glucose Transporters in Prenatally High-Glucose Exposed Three-Week Old Chickens Do Not Coincide with Aberrant Promoter DNA Methylation

doi: 10.1371/journal.pone.0119213

Figure Lengend Snippet: Relative gene expression of insulin receptor ( INSR ), leptin receptor (LEPR ), and glucose transporters 1 and 3 ( GLUT1 , GLUT3 ), all normalized to BETA ACTIN . Data are given as means ± SEM, shown as percentage of prenatally NaCl-treated controls. * p <0.05 (Mann-Whitney U-test)

Article Snippet: TaqMan gene expression assays: INSR : Gg03330786_m1, LEPR : Gg03347016_m1, GLUT1 : Gg03367103_m1, GLUT3 : Gg03349364_m1 (all FAM-labeled), and BETA ACTIN : Gg03815934_s1, VIC-labeled, primer limited.

Techniques: Gene Expression, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Acquired Alterations of Hypothalamic Gene Expression of Insulin and Leptin Receptors and Glucose Transporters in Prenatally High-Glucose Exposed Three-Week Old Chickens Do Not Coincide with Aberrant Promoter DNA Methylation

doi: 10.1371/journal.pone.0119213

Figure Lengend Snippet: Schematic illustration of sequence map of the glucose transporter 1 ( GLUT1 ) gene promoter region with chromosomal location of pyroassay (A) , and corresponding DNA methylation levels at individual CpG sites for prenatally NaCl-treated controls (black) and the prenatally glucose-treated group (gray) (B) .* p <0.05 (Mann-Whitney U-test)

Article Snippet: TaqMan gene expression assays: INSR : Gg03330786_m1, LEPR : Gg03347016_m1, GLUT1 : Gg03367103_m1, GLUT3 : Gg03349364_m1 (all FAM-labeled), and BETA ACTIN : Gg03815934_s1, VIC-labeled, primer limited.

Techniques: Sequencing, DNA Methylation Assay, MANN-WHITNEY